Volume 19, No. 5, 2022

Endophytic Bacteria Isolated From Bothriochloa Pertusa As An Ecological Alternative For Soil Cadmium Remediation

Alexander Pérez-Cordero ; Donicer E Montes-Vergara and Diego Carrillo-González


Phytoremediation is one of the current approaches for environmental pollution management. The combination of plant absorption of metals and endophyte bacteria which provide with ways to metabolize them is envisioned as a major technology for biorremediation. In this study, we studied Bothriochloa pertusa and their endophites’ ability to tolerate cadmium. We collected plants from different localities in Sucre, Colombia. Cadmium concentrations on soil and plant tissues were assesed. The density of endophytic bacteria from samples isolated from root and inflorescences was determined (CFU/g of tissue) and their capacity of tolerance to cadmium was evaluated in vitro. Cadmium was present in the soil at a concentration of 1.5±3.6 mg/Kg and from 1.05 to 3.21 mg/Kg (inflorescence and root respectively). Endophytic bacteria were present mostly in the inflorescence compared to the roots. Burkholderia diffusa KF475808 (95%) Burkholderia ambifaria LN88999 (98%); Deinococcus mumbaiensis DQ003135 (98%); Bacillus cereus GU056811 (93%); Bacillus toyonensis BCT-7112 (T) (99.9%); Pseudomonas fluorescens VI8L1 (98%) and Burkholderia cepacia KJ935921 (97%) tolerated up to 500 ppm of CdCl2 and showed in vitro capacity for nitrogen fixation, phosphate solubilization and the production of siderophores. This study demonstrates that endophytidc bacteria in B. pertusa have the potential to be used for fitoremediation of soil cadmium in the study area.

Pages: 133-147

Keywords: Bacteria, Grass, Soils, Sucre, Mojana, pollution, Colosuana.

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