Volume 18, No. 6, 2021

Structural And Compositional Characterization Of New Strain Of Geobacillus Stearothermophilus Under Heavy Metal-Induced Stress

Sonali Z. Patil and Geetha Unnikrishnan


Thermophiles can be found in almost any ecological niche from fresh and salt water to terrestrial and extreme environments, including metal-contaminated habitats. The efficiency of bioremediation by thermophiles is depended on the presence of biochemical factors present in the cell; like protein/ enzyme, EPS (Extra polymeric substances) or biogenic volatile substances. The first mechanisms by which bioremediation of toxic substances take place in thermophiles is with the help of protein/ enzymes. Structural and compositional characterization of new strain of Geobacillus stearothermophilus isolated from hot water spring under stressed and normal conditions were analysed. The band pattern in protein profiling showed the difference in pattern of protein expression in presence and absence of heavy metals. Some proteins were over expressed in presence of heavy metals and some new bands were formed that indicates important role of proteins/enzymes during stress conditions.

Pages: 2469-2475

Keywords: Thermophiles, protein profiling, Bioremediation, heavy Metals, Heavy metal induced stress

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